The main goal of this thesis is to explore the relationship between gender and language and to identify possible gender-determined differences between men and women. The thesis is separated into two parts, the theoretical part and the practical part. The theoretical part lists existing theories and previous research made in the field of gender and language. Also, it provides definitions and explanations for the key terms used in the rest of the thesis. The second part consists of the conducted research for this thesis. Data were collected through a questionnaire distributed to a group of male and female university students. To examine several elements of communication styles, including comfort level with direct communication, use of hedges, tag questions, polite speech forms, frequency of swearing, and perceptions of how gender influences communication style, the questionnaire comprised both closed-ended and open-ended questions. The results showed that there are several language differences between men and women and that gender roles impact the participants' communication styles. Consistent with traditional stereotypes of masculine communication, male participants were more likely to report using direct and assertive language, including humor and swearing. On the other hand, female participants frequently reported speaking more politely and shared worries about being perceived as negative when expressing opinions.