Based on the sex with which a person is born, society and culture impose certain expectations about the behavior and attitudes of that person using the process of socialization. Gender roles are used to conceptualize this mechanism. Our society reinforces adoption of traditional gender roles, which are in some studies found to have a negative effect on the wellbeing of women. Feminine gender roles stress is a response to situations that threaten internalized feminine standards. Feminine gender roles internalization refers to adoption of socially prescribed traditional gender roles and behavior. The aim of this study was to investigate the relationship between feminine gender roles stress, internalization of feminine gender roles and general wellbeing of women. The study included 225 women with the average of 29.65 years. Instruments used for this purpose were Feminine gender roles stress scale, Gender roles socialisation scale, Satisfaction with life scale, Positive and negative affect scale and General health questionnaire-12. The results show that feminine gender roles stress and feminine gender roles internalization negatively correlate with general wellbeing. The internalization of feminine gender roles has proven to be a significant predictor of well-being and significant moderator of the relationship between well-being and feminine gender roles stress. The effect wich feminine gender role stress and internalization of feminine gender roles have on the wellbeing of women points to the importance of women's experience and the need to involve experts in their investigation, analysis and application in society. These models have their application in feminist clinical psychology, implementation of preventive health care and educational programs as well as in organizational psychology.