The main objective of this study was to examine the effects of entrepreneurial education program on desirability of entrepreneurship, entrepreneurial self-efficacy and entrepreneurial intentions of students. Two-month program of entrepreneurial education (39 teaching hours), designed and conducted by the Faculty of Economics in Rijeka in collaboration with Primorje-Gorski Kotar County was attended by 16 participants (7 males and 9 females), students of integrated undergraduate and graduate courses at the University of Rijeka, age from 21 to 28. Measurement was carried out before and after the program, including the control group (equivalent couples), represented by 32 students, equal with participants by gender, age, faculty they attend, year of study and grade point average. Students from control group have not attended the program. Even though participants assess their entrepreneurial skills and abilities to be significantly better after the training, results indicate lower results on the scale of assessed desirability for entrepreneurship, compared to the initial measurement. Participant`s results on the scale of entrepreneurial self-efficacy and the scale of entrepreneurial intentions did not show statistically significant differences before and after the training. The results of the control group did not differ to the measurement time, which further confirms the effect of education, respectfully. Study additionally examined differences with regard to gender. Male students achieved significantly higher scores on all scales used in both measurements, compared to female students In addition to higher level of entrepreneurial tendencies and abilities, male students earned higher scores on the scale of desirability of entrepreneurship, entrepreneurial self-efficacy, entrepreneurial intentions scale and on level of development of entrepreneurial skills and abilities scale. Comparison of the students of economic and non-economic orientations, indicated that students of economic orientation achieved higher scores at one aspect of entrepreneurial potential (entrepreneurial awareness), as well as at desirability of entrepreneurship scale, entrepreneurial intentions scale, and at level of development of entrepreneurial skills and abilities scale. Although participants were generally satisfied with conducted educational program, expected positive changes in attitudes towards entrepreneurship, as well as the tendency to self-employment were not achieved. Given results lead to the whole series of issues related to further development of entrepreneurship training and increase of its effectiveness discussed in the field of business.