The harm reduction programme refers to a set of strategies, activities and ideas with the common goal of minimising harm and preventing risk behaviours, especially among young people related to sexual activity and psychoactive substance use (PAS). This paper focuses on harm reduction strategies and methods, with an emphasis on Croatia and neighbouring countries. Harm reduction is evolving because there is a growing global need for an effective way to prevent harm caused by risky behaviours, especially in communities where PAS are consumed. The principles of harm reduction are based on dignity and respect for human rights and aim to propose programmes that are effective, realistic and achievable. At the local level, we can see a huge need for harm reduction in the context of nightlife, but we can also see the improvement in the activities of NGOs seeking policy change in this area. The term “nightlife" encompasses all activities related to entertainment, recreation and socializing in the form of club parties, discos and festivals. Harm reduction practices in nightlife are never identical; they are unique and depend on many factors, such as the location and time of the event or the social profile of the party participants. Some of the activities are: On-site work, drug testing, training of event organizers and other nightlife employees, and testing for sexually transmitted diseases. It is important to emphasise that this paper also contributes to the implementation of harm reduction programmes in the young population.