There is a trend towards spending less and less time in nature and separation of people from the natural world. This is especially highlighted in the period of adolescence, during which levels of nature connectedness decrease, with many other challenges that can lead to a decrease in psychological well-being. This study aimed to investigate the relationship between nature connectedness and the subjective well-being of adolescents. Part of the research was carried out in secondary schools in Rijeka, and the second part was carried out during an experiential learning program in nature organized by "Outward Bound Croatia" in Lika. A total of 249 respondents between the ages of 15 and 19 participated, of which 35 participated in the OBC program. The new measure Nature Intelligence (Van den Berg and Albers, 2022) was used to examine the connection with nature and various aspects of subjective well-being were included in the research: life satisfaction, well-being index, positive and negative emotions, and general self-worth. The results of this research confirmed the positive correlation between the frequency of being in nature and nature connectedness, as well as the significant correlation of certain measures of subjective well-being with certain aspects of connection with nature. It has been shown that the experiential learning program in nature leads to an increase in the connection with nature, but not necessarily to an improvement in subjective well-being, except in lifesatisfaction. The contributions of motivation and the frequency of time spent in nature explaining the connection with nature were investigated, as well as the contributions of the experience of connection with nature and the frequency of being in nature in the explanation of subjective well-being. Most of the obtained results are in accordance with previous research, whereby this research provides new information about the relationship between different dimensions of nature connectedness and different aspects of the subjective well-being of adolescents. The conducted research can be used to create guidelines for designing programs in nature for adolescents, as well as a foundation for introducing changes in educational institutions, families and other communities with adolescents in the direction of encouraging and deepening their relationship with nature.